Molecular diagnostics are revolutionizing the clinical practice of infectious disease. Newer molecular technologies are promising in the identification of microorganisms
as well as evaluation of the presence of virulence factors and antibiotic resistance determinant. PCR is the most well-developed and proven molecular technique that
has a wide range of potential in clinical applications like specific or broad-spectrum pathogen detection, evaluation of emerging novel infections, surveillance,
early detection of bio threat agents, and antimicrobial resistance profiling. Early detection is essential to reduce the death rate and interrupt transmission.
The advantages of rapid turn-around time and high sensitivity and specificity are appealing for the shift of the traditional microbiological testing to molecular methods
in most of the cases.